Antibody responses to viral infections: a structural perspective across three different enveloped viruses Structural basis of pan-ebolavirus neutralization by an antibody targeting the glycoprotein fusion loop Analysis of a therapeutic antibody cocktail reveals determinants for cooperative and broad ebolavirus neutralization Considerations of antibody geometric constraints on NK cell antibody dependent cellular cytotoxicity Convergence of a common solution to broad ebolavirus neutralization by glycan cap directed human antibodies Protective pan-ebolavirus combination therapy by two multifunctional human antibodies Structural biology illuminates molecular determinants of broad ebolavirus neutralization by human antibodies for pan-ebolavirus therapeutic development Antibody therapeutics for the treatment of filoviral infection Mild hypoxia triggers transient blood-brain barrier disruption: A fundamental protective role for microglia. Cohesion-mediated loop anchors confine the location of human replication origins High throughput genome-wide single cell protein: DNA binding site mapping by targeted insertion of promoters (TIP-seq) Novel insights linking BRCA1-IRIS role in mammary gland development to formation of aggressive PABCs: The case for longer breastfeeding The molecular underpinning of geminin-overexpressing triple-negative breast cancer cells homing specifically to lungs. All of Dr. Takanori Otomo’s Publications All of Dr. Bruno Conti’s Publications Structural basis of ATG3 recognition by the autophagic ubiquitin-like protein ATG12. Structure of the human ATG12~ATG5 conjugate required for LC3 lipidation in autophagy. Insights into autophagosome biogenesis from structural and biochemical analyses of the ATG2A-WIPI4 complex The autophagic membrane tether ATG2A transfers lipids between membranes. Structure, lipid scrambling activity and role in autophagosome formation of ATG9A. Hot news about temperature and lifespan Two single nucleotide polymorphisms in IL13 and IL13RA1 from individuals with idiopathic Parkinson’s disease increase cellular susceptibility to oxidative stress. Insulin-like growth factor 1 receptor regulates hypothermia during calorie restriction. A Cool Way to Live Long Cutting Edge: IL-13Ralpha1 Expression in Dopaminergic Neurons Contributes to Their Oxidative Stress-Mediated Loss following Chronic Peripheral Treatment with Lipopolysaccharide. Transgenic mice with a reduced core body temperature have an increased life span. High throughput genome-wide single cell protein: DNA binding site mapping by targeted insertion of promoters (TIP-seq) Replication timing maintains the global epigenetic state in human cells Genome-Wide Identification of Early-Firing Human Replication Origins by Optical Replication Mapping The Multiple Pathways to Autoimmunity 3D genome organization contributes to genome instability at fragile sites High Resolution Repli-Seq defines the temporal choreography of initiation, elongation, and termination of replication in mammalian cells. Replication timing alterations in leukemia affect clinically relevant chromosome domains Control of DNA replication timing in the 4D genome Identifying cis-Elements for Spatiotemporal Control of Mammalian DNA Replication Early differentiation of human embryonic stem cells is accompanied by rapid transcriptional re-programming and discordance between replication timing and chromatin compartment All of Dr. Dave Gilbert’s Publications Reactive oxygen species regulate protrusion efficiency by controlling actin dynamics A novel Rac1 GAP splice variant relays poly-Ub accumulation signals to mediate Rac2 inactivation Hypoxia in multiple sclerosis; is it the chicken or the egg? Activated Protein C attenuates experimental autoimmune encephalomyelitis progression by enhancing vascular integrity and suppressing microglial activation Chronic mild hypoxia accelerates recovery from preexisting EAE by enhancing vascular integrity and apoptosis of infiltrated monocytes Mild hypoxia triggers transient blood-brain barrier disruption: a fundamental role for microglia The pro- and anti-tumor roles of mesenchymal stem cells toward BRCA1-IRIS-overexpressing TNBC cells. Targeting AXL and RAGE to prevent geminin overexpression-induced triple-negative breast cancer metastasis CD4+CD25+CD127hi cell frequency predicts disease progression in type 1 diabetes Anti-IFN-alpha/beta receptor antibody treatment ameliorates disease in lupus-predisposed mice Lupus acceleration by a MAVS-activating RNA virus requires endosomal TLR signaling and host genetic predispostion All of Dr. Roberto Baccala’s Publications Essential requirement for IRF8 and SLC5A4 implicates plasmacytoid dendritic cells in the pathogenesis of lupus Type-1 interferon receptor deficiency reduces lupus-like disease in NZB mice Silica exposure and chronic virus infection synergistically promote lupus-like systemic autoimmunity in mice with low genetic predisposition TLR-dependent and TLR-independent pathways of type I interferon induction in systemic autoimmunity Type I interferon is a therapeutic target for virus-induced lethal vascular damage Immune checkpoint modulation enhances HIV-1 antibody induction Targeted Selection of HIV-specific Antibody Mutations by Engineering B Cell Maturation Cross-Reactivity to Kynureninase Tolerizes B Cells That Express the HIV-1 Broadly Neutralizing Antibody 2F5 The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template Vaccine Induction of Heterologous Tier 2 HIV-1 Neutralizing Antibodies in Animal Models Chronic mild hypoxia increases expression of laminins 111 and 411 and the laminin receptor α6β1 integrin at the blood-brain barrier Absence of endothelial α5β1 integrin triggers early onset of experimental autoimmune encephalomyelitis due to reduced vascular remodeling and compromised vascular integrity A critical role for microglia in maintaining vascular integrity in the hypoxic spinal cord Sirtuin 1-Chromatin-Binding Dynamics Points to a Common Mechanism Regulating Inflammatory Targets in SIV Infection and in the Aging Brain Brain Reward Function After Chronic and Binge Methamphetamine Regimens in Mice Expressing the HIV-1 TAT Protein Systems Biology Analysis of the Antagonizing Effects of HIV-1 Tat Expression in the Brain over Transcriptional Changes Caused by Methamphetamine Sensitization The Immunosuppressive Microenvironment in BRCA1-IRIS-Overexpressing TNBC Tumors Is Induced by Bidirectional Interaction with Tumor-Associated Macrophages Engineering Pak1 Allosteric Switches Inhibition of RhoA reduces propofol-mediated growth cone collapse, axonal transport impairment, loss of synaptic connectivity, and behavioural deficits Caveolin-1 Phosphorylation Is Essential for Axonal Growth of Human Neurons Derived From iPSCs Cancer-driven changes link T cell frequency to muscle strength in people with cancer: a pilot study Glycoengineering HIV-1 Env creates ‘supercharged’ and ‘hybrid’ glycans to increase neutralizing antibody potency, breadth and saturation Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Envelope Cancer-driven changes link T cell frequency to muscle strength in people with cancer: a pilot study. Tjitro R, Campbell LA, Basova L, Johnson J, Najera JA, Lindsey A and Marcondes MC Modeling the Function of TATA Box Binding Protein in Transcriptional Changes Induced by HIV-1 Tat in Innate Immune Cells and the Effect of Methamphetamine Exposure. Front. Immunol. 9:3110. doi: 10.3389/fimmu.2018.03110 All of Dr. Celine DerMardirossian’s Publications Bielek, H., Anselmo, A., & Dermardirossian, C. (2009). Morphological and proliferative abnormalities in renal mesangial cells lacking RhoGDI. Cellular signalling, 21(12), 1974-83. DerMardirossian, C., Rocklin, G., Seo, J. Y., & Bokoch, G. M. (2006). Phosphorylation of RhoGDI by Src regulates Rho GTPase binding and cytosol-membrane cycling. Molecular biology of the cell, 17(11), 4760-8. DerMardirossian C, Schnelzer A, Bokoch GM. Mol Cell. 2004 Jul 2;15(1):117-27. Pathak, R., Delorme-Walker, V. D., Howell, M. C., Anselmo, A. N., White, M. A., Bokoch, G. M., & Dermardirossian, C. (2012). The microtubule-associated Rho activating factor GEF-H1 interacts with exocyst complex to regulate vesicle traffic. Developmental cell, 23(2), 397-411. Hodgson, L., Spiering, D., Sabouri-Ghomi, M., Dagliyan, O., DerMardirossian, C., Danuser, G., & Hahn, K. M. (2016). FRET binding antenna reports spatiotemporal dynamics of GDI-Cdc42 GTPase interactions. Nature chemical biology, 12(10), 802-809. Huang, T. Y., Michael, S., Xu, T., Sarkeshik, A., Moresco, J. J., Yates, J. R., Masliah, E., Bokoch, G. M., … DerMardirossian, C. (2013). A novel Rac1 GAP splice variant relays poly-Ub accumulation signals to mediate Rac1 inactivation. Molecular biology of the cell, 24(3), 194-209. Pathak, R., & Dermardirossian, C. (2013). GEF-H1: orchestrating the interplay between cytoskeleton and vesicle trafficking. Small GTPases, 4(3), 174-9. Delorme-Walker, V., Seo, J. Y., Gohla, A., Fowler, B., Bohl, B., & DerMardirossian, C. (2015). Chronophin coordinates cell leading edge dynamics by controlling active cofilin levels. Proceedings of the National Academy of Sciences of the United States of America, 112(37), E5150-9. Chronic mild hypoxia promotes profound vascular remodeling in spinal cord blood vessels, preferentially in white matter, via an α5β1 integrin-mediated mechanism Boroujerdi, A., Welser-Alves, J. V. & Milner, R. (2013). Extensive vascular remodeling in the spinal cord of pre-symptomatic experimental autoimmune encephalomyelitis mice; increased vessel expression of fibronectin and the a5b1 integrin. Exp. Neurol. 250, 43-51. Welser, J., Halder, S. K., Kant, R., Boroujerdi, A. and Milner, R. (2017). Endothelial a6b4 integrin protects during experimental autoimmune encephalomyelitis-induced neuroinflammation by maintaining vascular integrity and tight junction protein expression. J. Neuroinflamm. 14, 217. Boroujerdi, A. and Milner, R. (2015). Defining the critical hypoxic threshold that promotes vascular remodeling in the brain. Exp. Neurol. 263, 132-140. Halder, S. K., Kant, R. and Milner, R. (2018). Hypoxic pre-conditioning suppresses experimental autoimmune encephalomyelitis by modifying multiple properties of blood vessels. Acta. Neuropathologica. Communications. 6, 86. All of Dr. Richard Milner’s Publications Dopamine and its receptors play a role in the modulation of CCR5 expression in innate immune cells following exposure to Methamphetamine: Implications to HIV infection Basova L, Najera JA, Bortel N, Wang D, Moya R, Alexander L, Semenova S, Ellis RJ, Marcondes M. All of Dr. Wael ElShamy’s Publications All of Dr. Marilyn Diaz’s Publications Swift entry of myelin-specific T lymphocytes into the central nervous system in spontaneous autoimmune encephalomyelitis. Immune regulatory mechanisms influence early pathology in spinal cord injury and in spontaneous autoimmune encephalomyelitis IFN-gamma determines distinct clinical outcomes in autoimmune encephalomyelitis The role of IFN-gamma in the production of Th2 subpopulations: implications for variable Th2-mediated pathologies in autoimmunity All of Dr. Barbara Mueller’s Publications All of Dr. Laurent Verkoczy’s Publications All of Dr. Fahumiya Samad’s Publications All of Dr. Maria Cecilia Marcondes’ Publication All of Dr. James Binley’s Publications All of Dr. Joanna Davies’ Publications Sirtuin 1-Chromatin-Binding Dynamics Points to a Common Mechanism Regulating Inflammatory Targets in HIV Infection and in the Aging Brain HIV-1 TAT protein enhances sensitization to methamphetamine by affecting dopaminergic function The plasminogen receptor, Plg-RKT, is essential for mammary lobuloalveolar development and lactation Identification of the coagulation factor VIIa integrin binding site required for pro-angiogenic PAR2 signaling Deficiency of plasminogen receptor, Plg-RKT, causes defects in plasminogen binding and inflammatory macrophage recruitment in vivo A niche that triggers aggressiveness within BRCA1-IRIS overexpressing triple negative tumors is supported by reciprocal interactions with the microenvironment Memory T cells in Type 1 Diabetes: The Devil is in the Detail Human CD4+ CD25+ CD127hi cells and the Th1/Th2 phenotype Effects of partially dismantling the CD4 binding site glycan fence of HIV-1 Envelope glycoprotein trimers on neutralizing antibody induction. Virus-like Particles Identify an HIV V1V2 Apex-Binding Neutralizing Antibody that Lacks a Protruding Loop Glycoengineering HIV-1 Env Creates ‘Supercharged’ and ‘Hybrid’ Glycans to Increase Neutralizing Antibody Potency, Breadth and Saturation. The Secret to Raising Resilient Kids The Newest Brain Wave in Epilepsy Care A rough guide to advisory boards Canadian Guidelines for the Management of Plaque Psoriasis Effectiveness of live telephone support in improving haemophilia clinic attendance. Joining the patient on the path to customized prophylaxis: one hemophilia team explores the tools of engagement Altered Ig hypermutation pattern and frequency in complementary mouse models of DNA polymerase ζ activity. Altered pattern of immunoglobulin hypermutation in mice deficient in Slip-GC prote The role of activation-induced deaminase in lupus nephritis A smad signaling network regulates islet cell proliferation Autoreactivity in HIV-1 broadly neutralizing antibodies: implications for their function and induction by vaccination HIV-1 envelope gp41 broadly neutralizing antibodies: hurdles for vaccine development Host controls of HIV neutralizing antibodies. Science Modulation of nonneutralizing HIV-1 gp41 responses by an MHC-restricted TH epitope overlapping those of membrane proximal external region broadly neutralizing antibodies. Apoptotic Debris Accumulates on Hematopoietic Cells and Promotes Disease in Murine and Human Systemic Lupus Erythematosus Initiation of immune tolerance-controlled HIV gp41 neutralizing B cell lineages. Humanized Immunoglobulin Mice: Models for HIV Vaccine Testing and Studying the Broadly Neutralizing Antibody Problem Initiation of HIV neutralizing B cell lineages with sequential envelope immunizations. Phenotypic changes in the brain of SIV-infected macaques exposed to methamphetamine parallel macrophage activation patterns induced by the common gamma-chain cytokine system The cross-talk of HIV-1 Tat and methamphetamine in HIV-associated neurocognitive disorders In vivo effects of methamphetamine on brown fat reactive oxygen species and mitochondria Astrocyte-specific overexpressed gene signatures in response to methamphetamine exposure in vitro Methamphetamine abuse affects gene expression in brain-derived microglia of SIV-infected macaques to enhance inflammation and promote virus targets BRCA1-IRIS inactivation overcomes paclitaxel resistance in triple negative breast cancers Geminin overexpression promotes imatinib sensitive breast cancer: a novel treatment approach for aggressive breast cancers, including a subset of triple negative Geminin overexpression prevents the completion of topoisomerase IIα chromosome decatenation, leading to aneuploidy in human mammary epithelial cells Geminin overexpression-dependent recruitment and crosstalk with mesenchymal stem cells enhance aggressiveness in triple negative breast cancers BRCA1-IRIS overexpression promotes and maintains the tumor initiating phenotype: implications for triple negative breast cancer early lesions A novel role for CD4+ T cells in the control of cachexia. Cachexia in the non obese diabetic mouse is associated with CD4+ T cell lymphopenia. Distribution and regulation of plasminogen activator inhibitor-1 in murine adipose tissue in vivo: Induction by tumor necrosis factor-a and lipopolysaccharide. Tumor necrosis factor-a: A key component in the obesity-linked elevation of plasminogen activator inhibitor-1. Keratinocyte-derived Chemokine in Obesity: Expression and Role in Adipose Macrophage Infiltration and Glucose homeostasis. Tissue factor-protease activated receptor 2 signaling promotes diet-induced obesity and adipose inflammation. Sphingosine kinase 1 regulates adipose proinflammatory responses and insulin resistance. Tissue factor expression in obese type 2 diabetic subjects and its regulation by antidiabetic agents. Enzyme digests eliminate nonfunctional Env from HIV-1 particle surfaces, leaving native Env trimers intact and viral infectivity unaffected. HIV-1 virus-like particles bearing pure env trimers expose neutralizing epitopes but occlude nonneutralizing epitopes. Multi-parameter exploration of HIV-1 virus-like particles as neutralizing antibody immunogens in guinea pigs, rabbits and macaques. Use of enzyme-digested virus-like particles as probes for flow cytometric sorting of HIV-specific neutralizing Ab-producing B-cells. AIDS Res Hum Retroviruses. Vaccine-Elicited Tier 2 HIV-1 Neutralizing Antibodies Bind to Quaternary Epitopes Involving Glycan-Deficient Patches Proximal to the CD4 Binding Site. A peripheral CD4+ T cell precursor for naïve, memory and regulatory T cells. High-throughput sequencing of islet-infiltrating memory CD4+ T cells reveals a similar pattern of TCR Vβ usage in prediabetic and diabetic NOD mice. T cell populations in the pancreatic lymph node naturally and consistently expand and contract in NOD mice as disease progresses. Requirement for binding of catalytically active factor VIIa in tissue factor dependent experimental metastasis. Activation of endothelial cell protease activated receptor 1 by the protein C pathway. Regulation of angiogenesis by tissue factor cytoplasmic domain signaling. Local adipocytes enable estrogen-dependent breast cancer growth: Role of leptin and aromatase. Endothelial Protein C Receptor Function in Murine and Human Breast Cancer Development. Deficiency of plasminogen receptor, Plg-RKT , causes defects in plasminogen binding and inflammatory macrophage recruitment in vivo. New insights into the role of Plg-RKT in macrophage recruitment. CD4+ CD44v.low cells are unique peripheral precursors that are distinct from recent thymic emigrants and stem cell-like memory cells. A pilot study showing associations between frequency of CD4+ memory cell subsets at diagnosis and duration of partial remission in type 1 diabetes

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