Flow Cytometry with CytoFlex S: The Basics

Our second virtual science focus meeting was hosted by Aditi Narsale, Ph.D. who presented on a technique called flow cytometry that makes use of a machine called, CytoFlex S. Almost every research group at SDBRI has some interest in using flow cytometry for their research. Dr. Narsale gave a refresher course on the basics of this complicated technology including, but not limited to, lasers types, staining protocol, detecting size, shape, and granularity, and identifying and quantifying extracellular vesicles (EV) by flow cytometry. In the Q&A portion following her seminar, she helped to troubleshoot and solve problems highlighted by other institute members that relate to flow cytometry.