Allosteric Anti-KLK4 Antibody Development for Targeted Anti-cancer Effects in Ovarian Carcinoma

Adjunct Professor Ashley Buckle is excited to share their latest publication in the Journal of Molecular Biology:

https://www.sciencedirect.com/science/article/abs/pii/S0022283625001676

Dr. Buckle and the team involved has developed a highly specific monoclonal antibody targeting an allosteric region of KLK4, a serine protease implicated in ovarian carcinoma progression. By combining structural biology, innovative immunization strategies, and computational modeling, they identified and characterized an antibody (ABS4) that not only effectively inhibits KLK4’s enzymatic activity but also significantly reduces proliferation and migration in aggressive ovarian cancer cells.

This was a long-term collaboration with the lab of Irit Sagi, and the work was led by Nikolaos Afratis, who is now leading his own research group at the University of Athens.

Congratulations to all!